Single-cell multimodal technologies are becoming the hot topic of single-cell heterogeneity and function studies, promising to unravel the hidden relationship and functionalities of different aspects of the cells. Among the plethora of single-cell technologies, interesting is the patch-seq technology, which simultaneously performs Patch clamp measures and scRNA-seq on the same cells. However, given the experimental limitations of throughput of Patch clamp, the scRNA-seq analysis is challenging because it requires more samples to investigate cellular heterogeneity. Usually, the solution is associating the cells with the cell types in an existing scRNA-seq dataset. However, doing so loses part of the single cell resolution of the multimodal technique. Therefore, this work proposes a procedure leveraging the Seurat Integration process to find from a reference dataset t he most similar cells to the ones from the patch-seq. The similarity is how much gene expression profiles are identical, and to evaluate that, this work defines various etrics based on R and Index. In this way, one obtains a selection of suitable Reference cells to enrich the number of cells on which to perform multimodal investigation.

High-resolution sample size enrichment of single-cell multi-modal low-throughput Patch-seq datasets / Martini, Lorenzo; Bardini, Roberta; Savino, Alessandro; Di Carlo, Stefano. - ELETTRONICO. - (2022), pp. 2334-2341. (Intervento presentato al convegno 2022 IEEE International Conference on Bioinformatics and Biomedicine (BIBM) tenutosi a Las Vegas (USA) nel Dec. 6-8, 2022) [10.1109/BIBM55620.2022.9995529].

High-resolution sample size enrichment of single-cell multi-modal low-throughput Patch-seq datasets

Martini, Lorenzo;Bardini, Roberta;Savino, Alessandro;Di Carlo, Stefano
2022

Abstract

Single-cell multimodal technologies are becoming the hot topic of single-cell heterogeneity and function studies, promising to unravel the hidden relationship and functionalities of different aspects of the cells. Among the plethora of single-cell technologies, interesting is the patch-seq technology, which simultaneously performs Patch clamp measures and scRNA-seq on the same cells. However, given the experimental limitations of throughput of Patch clamp, the scRNA-seq analysis is challenging because it requires more samples to investigate cellular heterogeneity. Usually, the solution is associating the cells with the cell types in an existing scRNA-seq dataset. However, doing so loses part of the single cell resolution of the multimodal technique. Therefore, this work proposes a procedure leveraging the Seurat Integration process to find from a reference dataset t he most similar cells to the ones from the patch-seq. The similarity is how much gene expression profiles are identical, and to evaluate that, this work defines various etrics based on R and Index. In this way, one obtains a selection of suitable Reference cells to enrich the number of cells on which to perform multimodal investigation.
2022
978-1-6654-6819-0
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2974693