Archivio Istituzionale della RicercaThe evaluation of the chlorophyll content in fruit peel is usually performed by means of complex destructive procedures that include extraction and separation. In the present work, a non-destructive procedure, based on UV-Vis spectroscopy in the diffuse reflectance mode, is proposed for the quantitative measurement of both chlorophylls a and b, utilising the spectral range in which molecules other than chlorophylls show low absorbance. Two methods are presented and compared, one based on multivariate analysis over the whole range, and the other based on a linear regression at two meaningful wavelengths.
Fast non-destructive determination of chlorophylls in apple skin / Venturello, Alberto; Ceccarelli, Raffaela; Garrone, Edoardo; Geobaldo, Francesco. - In: ITALIAN JOURNAL OF FOOD SCIENCE. - ISSN 1120-1770. - STAMPA. - 24:2(2012), pp. 167-172.
Fast non-destructive determination of chlorophylls in apple skin
VENTURELLO, ALBERTO;CECCARELLI, RAFFAELA;GARRONE, EDOARDO;GEOBALDO, FRANCESCO
2012
Abstract
The evaluation of the chlorophyll content in fruit peel is usually performed by means of complex destructive procedures that include extraction and separation. In the present work, a non-destructive procedure, based on UV-Vis spectroscopy in the diffuse reflectance mode, is proposed for the quantitative measurement of both chlorophylls a and b, utilising the spectral range in which molecules other than chlorophylls show low absorbance. Two methods are presented and compared, one based on multivariate analysis over the whole range, and the other based on a linear regression at two meaningful wavelengths.
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https://hdl.handle.net/11583/2467784
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