The use of biomaterials and scaffolds to boost bone regeneration is increasingly gaining interest as a complementary method to the standard surgical and pharmacological treatments in case of severe injuries and pathological conditions. In this frame, the selection of biomaterials and the accurate assessment of the manufacturing procedures are considered key factors in the design of constructs able to resemble the features of the native tissue and effectively induce specific cell responses. Accordingly, composite scaffolds based on type-I-collagen can mimic the composition of bone extracellular matrix (ECM), while electrospinning technologies can be exploited to produce nanofibrous matrices to resemble its architectural organization. However, the combination of collagen and electrospinning reported several complications due to the frequent denaturation of the protein and the variability of results according to collagen origin, concentration, and solvent. In this context, the strategies optimized in this study enabled the preparation of collagen-based electrospun scaffolds characterized by about 100 nm fibers, preserving the physico-chemical properties of the protein thanks to the use of an acetic acid-based solvent. Moreover, nanoparticles of mesoporous bioactive glasses were combined with the optimized collagen formulation, proving the successful design of composite scaffolds resembling the morphological features of bone ECM at the nanoscale.

Biomimetic scaffolds obtained by electrospinning of collagen-based materials: Strategies to hinder the protein denaturation / Montalbano, G.; Tomasina, C.; Fiorilli, S.; Camarero-Espinosa, S.; Vitale Brovarone, C.; Moroni, L.. - In: MATERIALS. - ISSN 1996-1944. - ELETTRONICO. - 14:16(2021), p. 4360. [10.3390/ma14164360]

Biomimetic scaffolds obtained by electrospinning of collagen-based materials: Strategies to hinder the protein denaturation

Montalbano G.;Fiorilli S.;Vitale Brovarone C.;
2021

Abstract

The use of biomaterials and scaffolds to boost bone regeneration is increasingly gaining interest as a complementary method to the standard surgical and pharmacological treatments in case of severe injuries and pathological conditions. In this frame, the selection of biomaterials and the accurate assessment of the manufacturing procedures are considered key factors in the design of constructs able to resemble the features of the native tissue and effectively induce specific cell responses. Accordingly, composite scaffolds based on type-I-collagen can mimic the composition of bone extracellular matrix (ECM), while electrospinning technologies can be exploited to produce nanofibrous matrices to resemble its architectural organization. However, the combination of collagen and electrospinning reported several complications due to the frequent denaturation of the protein and the variability of results according to collagen origin, concentration, and solvent. In this context, the strategies optimized in this study enabled the preparation of collagen-based electrospun scaffolds characterized by about 100 nm fibers, preserving the physico-chemical properties of the protein thanks to the use of an acetic acid-based solvent. Moreover, nanoparticles of mesoporous bioactive glasses were combined with the optimized collagen formulation, proving the successful design of composite scaffolds resembling the morphological features of bone ECM at the nanoscale.
File in questo prodotto:
File Dimensione Formato  
Biomimetic Scaffolds Obtained by Electrospinning of Collagen-Based Materials_ Strategies to Hinder the Protein Denaturation_Materials 2021.pdf

non disponibili

Descrizione: Articolo elettronico open access
Tipologia: 2a Post-print versione editoriale / Version of Record
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 20.34 MB
Formato Adobe PDF
20.34 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
pdfresizer.com-pdf-resize.pdf

accesso aperto

Descrizione: Versione ridimensionata
Tipologia: 2a Post-print versione editoriale / Version of Record
Licenza: Creative commons
Dimensione 5.89 MB
Formato Adobe PDF
5.89 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2949380