In vitro and in vivo studies are fundamental steps in the characterization of new im-plantable materials to preliminarily assess their biological response. The present study reports the in vitro and in vivo characterizations of a novel experimental silicate bioactive glass (BG) (47.5 B, 47.5 SiO2-10 Na2O-10 K2O-10 MgO-20 CaO-2.5 P2O5 mol.%). Cytocompatibility tests were perfor-med using human mature osteoblasts (U2OS), human mesenchymal stem cells (hMSCs) and human endothelial cells (EA.hy926). The release of the early osteogenic alkaline phosphatase (ALP) marker suggested strong pro-osteogenic properties, as the amount was comparable between hMSCs cultivated onto BG surface and cells cultivated onto polystyrene control. Similarly, real-time PCR revealed that the osteogenic collagen I gene was overexpressed in cells cultivated onto BG surface without biochemical induction. Acute toxicity tests for the determination of the median lethal dose (LD50 ) al-lowed classifying the analyzed material as a slightly toxic substance with LD50 = 4522 ± 248 mg/kg. A statistically significant difference in bone formation was observed in vivo through comparing the control (untreated) group and the experimental one, proving a clear osteogenic effect induced by the implantation at the defect site. Complete resorption of 47.5 B powder was observed after only 3 months in favor of newly formed tissue, thus confirming the high osteostimulatory potential of 47.5 B glass.
Biological evaluation of a new sodium-potassium silico-phosphate glass for bone regeneration: In vitro and in vivo studies / Fiume, E.; Tulyaganov, D. U.; Akbarov, A.; Ziyadullaeva, N.; Cochis, A.; Scalia, A. C.; Rimondini, L.; Verne', E.; Baino, F.. - In: MATERIALS. - ISSN 1996-1944. - ELETTRONICO. - 14:16(2021), p. 4546. [10.3390/ma14164546]
|Titolo:||Biological evaluation of a new sodium-potassium silico-phosphate glass for bone regeneration: In vitro and in vivo studies|
|Data di pubblicazione:||2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ma14164546|
|Appare nelle tipologie:||1.1 Articolo in rivista|