Physical stimuli are crucial for the structural and functional maturation of tissues both in vivo and in vitro . In tissue engineering applications, bioreactors have become fundamental and effective tools for provid- ing biomimetic culture conditions that recapitulate the native physical stimuli. In addition, bioreactors play a key role in assuring strict control, automation, and standardization in the production process of cell-based products for future clinical application. In this study, a compact, easy-to-use, tunable stretch bioreactor is proposed. Based on customizable and low-cost technological solutions, the bioreactor was designed for providing tunable mechanical stretch for biomimetic dynamic culture of different engineered tissues. In-house validation tests demonstrated the accuracy and repeatability of the imposed mechanical stimulation. Proof of concepts biological tests performed on engineered cardiac constructs, based on de- cellularized human skin scaffolds seeded with human cardiac progenitor cells, confirmed the bioreactor Good Laboratory Practice compliance and ease of use, and the effectiveness of the delivered cyclic stretch stimulation on the cardiac construct maturation.

Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs / Putame, Giovanni; Gabetti, Stefano; Carbonaro, Dario; Di Meglio, Franca; Romano, Veronica; Maria Sacco, Anna; Belviso, Immacolata; Serino, Gianpaolo; Bignardi, Cristina; Morbiducci, Umberto; Castaldo, Clotilde; Massai, DIANA NADA CATERINA. - In: MEDICAL ENGINEERING & PHYSICS. - ISSN 1873-4030. - ELETTRONICO. - 84:(2020). [10.1016/j.medengphy.2020.07.018]

Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs

Giovanni Putame;Stefano Gabetti;Dario Carbonaro;Gianpaolo Serino;Cristina Bignardi;Umberto Morbiducci;Diana Massai
2020

Abstract

Physical stimuli are crucial for the structural and functional maturation of tissues both in vivo and in vitro . In tissue engineering applications, bioreactors have become fundamental and effective tools for provid- ing biomimetic culture conditions that recapitulate the native physical stimuli. In addition, bioreactors play a key role in assuring strict control, automation, and standardization in the production process of cell-based products for future clinical application. In this study, a compact, easy-to-use, tunable stretch bioreactor is proposed. Based on customizable and low-cost technological solutions, the bioreactor was designed for providing tunable mechanical stretch for biomimetic dynamic culture of different engineered tissues. In-house validation tests demonstrated the accuracy and repeatability of the imposed mechanical stimulation. Proof of concepts biological tests performed on engineered cardiac constructs, based on de- cellularized human skin scaffolds seeded with human cardiac progenitor cells, confirmed the bioreactor Good Laboratory Practice compliance and ease of use, and the effectiveness of the delivered cyclic stretch stimulation on the cardiac construct maturation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2842487