Archivio Istituzionale della RicercaAn innovative, low-cost, 3D-printed sample-holder is proposed for reproducible and effective stirring-based decellularization of biological tissues. The sample-holder was designed to be low-cost, easy to use with conventional laboratory equipment, and manufacturable through 3D printing. During stirring-based decellularization, the sample holder exposes the samples to convective flow, enhancing the reagent transport while protecting the samples from disruptive forces. Computational fluid dynamics analyses were carried out to elucidate the developing hydrodynamics. Explanatory tests, performed on human cardiac tissue samples, demonstrated the effectiveness of the presented device.
A low-cost 3D-printed sample-holder for stirring-based decellularization of biological tissues / Carbonaro, Dario; Putame, Giovanni; Siciliano, Katia; Castaldo, Clotilde; Di Meglio, Franca; Belviso, Immacolata; Maria Sacco, Anna; Morbiducci, Umberto; Gallo, Diego; Massai, DIANA NADA CATERINA; Audenino, Alberto. - ELETTRONICO. - (2019), pp. 40-41. (Intervento presentato al convegno IX Annual Meeting of the Italian Chapter of the European Society of Biomechanics (ESB-ITA) tenutosi a Bologna).
A low-cost 3D-printed sample-holder for stirring-based decellularization of biological tissues
Dario Carbonaro;Giovanni Putame;Katia Siciliano;Umberto Morbiducci;Diego Gallo;Diana Massai;Alberto Audenino
2019
Abstract
An innovative, low-cost, 3D-printed sample-holder is proposed for reproducible and effective stirring-based decellularization of biological tissues. The sample-holder was designed to be low-cost, easy to use with conventional laboratory equipment, and manufacturable through 3D printing. During stirring-based decellularization, the sample holder exposes the samples to convective flow, enhancing the reagent transport while protecting the samples from disruptive forces. Computational fluid dynamics analyses were carried out to elucidate the developing hydrodynamics. Explanatory tests, performed on human cardiac tissue samples, demonstrated the effectiveness of the presented device.
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https://hdl.handle.net/11583/2762112
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