Archivio Istituzionale della RicercaWe report on the fabrication of an elastomeric multichamber microfluidic device hosting SERS active silvercoated porous silicon membranes. The chip, characterized in terms of Raman efficiency (enhancements > 10 7) and uniformity using 4-mercaptobenzoic acid (4-MBA) as non-resonant probe molecule, allows calibration of the SERS signal intensity vs. the analyte concentration. After a suitable optimization of a functionalization protocol the device is applied for the label free detection of microRNA tumor markers in a hybridization based biosensing assay.
Ag-COATED POROUS SILICON-PDMS MEMBRANES INTEGRATED IN A SERS MICROFLUIDIC DEVICE FOR LABEL FREE DETECTION OF MICRO-RNA / Novara, Chiara; Chiado', Alessandro; Lamberti, Andrea; Rivolo, Paola; Geobaldo, Francesco; Giorgis, Fabrizio. - STAMPA. - (2016), pp. 394-395. (Intervento presentato al convegno PSST 2016- 10th International Conference on Porous Semiconductors science and Technology tenutosi a Tarragona (ES) nel 6-11 March 2016).
Ag-COATED POROUS SILICON-PDMS MEMBRANES INTEGRATED IN A SERS MICROFLUIDIC DEVICE FOR LABEL FREE DETECTION OF MICRO-RNA
NOVARA, CHIARA;CHIADO', ALESSANDRO;LAMBERTI, ANDREA;RIVOLO, PAOLA;GEOBALDO, FRANCESCO;GIORGIS, FABRIZIO
2016
Abstract
We report on the fabrication of an elastomeric multichamber microfluidic device hosting SERS active silvercoated porous silicon membranes. The chip, characterized in terms of Raman efficiency (enhancements > 10 7) and uniformity using 4-mercaptobenzoic acid (4-MBA) as non-resonant probe molecule, allows calibration of the SERS signal intensity vs. the analyte concentration. After a suitable optimization of a functionalization protocol the device is applied for the label free detection of microRNA tumor markers in a hybridization based biosensing assay.
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https://hdl.handle.net/11583/2643716
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