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EnglishThis paper presents an automated algorithm to determine DNA fragment size from atomic force microscope images and to extract the molecular profiles. The sizing of DNA fragments is a widely used procedure for investigating the physical properties of individual or protein-bound DNA molecules. Several atomic force microscope (AFM) real and computer-generated images were tested for different pixel and fragment sizes and for different background noises. The automated approach minimizes processing time with respect to manual and semi-automated DNA sizing. Moreover, the DNA molecule profile recognition can be used to perform further structural analysis. For computer-generated images, the root mean square error incurred by the automated algorithm in the length estimation is 0.6% for a 7.8 nm image pixel size and 0.34% for a 3.9 nm image pixel size. For AFM real images we obtain a distribution of lengths with a standard deviation of 2.3% of mean and a measured average length very close to the real one, with an error around 0.33%.
Automated DNA Fragments Recognition and Sizing through AFM Image Processing / FICARRA E.; BENINI L; MACII E; ZUCCHERI G. - In: IEEE TRANSACTIONS ON INFORMATION TECHNOLOGY IN BIOMEDICINE. - ISSN 1089-7771. - 9(4)(2005), pp. 508-517.
| Titolo: | Automated DNA Fragments Recognition and Sizing through AFM Image Processing |
| Autori: | |
| Data di pubblicazione: | 2005 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1109/TITB.2005.855546 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| draftTITB_2005.pdf | 1. Preprint / Submitted Version | PUBBLICO - Tutti i diritti riservati | Visibile a tuttiVisualizza/Apri |
http://hdl.handle.net/11583/1501829
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