This paper presents an automated algorithm to determine DNA fragment size from atomic force microscope images and to extract the molecular profiles. The sizing of DNA fragments is a widely used procedure for investigating the physical properties of individual or protein-bound DNA molecules. Several atomic force microscope (AFM) real and computer-generated images were tested for different pixel and fragment sizes and for different background noises. The automated approach minimizes processing time with respect to manual and semi-automated DNA sizing. Moreover, the DNA molecule profile recognition can be used to perform further structural analysis. For computer-generated images, the root mean square error incurred by the automated algorithm in the length estimation is 0.6% for a 7.8 nm image pixel size and 0.34% for a 3.9 nm image pixel size. For AFM real images we obtain a distribution of lengths with a standard deviation of 2.3% of mean and a measured average length very close to the real one, with an error around 0.33%.
Automated DNA Fragments Recognition and Sizing through AFM Image Processing / FICARRA E.; BENINI L; MACII E; ZUCCHERI G. - In: IEEE TRANSACTIONS ON INFORMATION TECHNOLOGY IN BIOMEDICINE. - ISSN 1089-7771. - 9(4):(2005), pp. 508-517. [10.1109/TITB.2005.855546]
|Titolo:||Automated DNA Fragments Recognition and Sizing through AFM Image Processing|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1109/TITB.2005.855546|
|Appare nelle tipologie:||1.1 Articolo in rivista|