Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.
Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles / Paxinou, Alexandra; Marcello, Elena; Vecchiato, Vittoria; Erman, Lara; Wright, Edward; Noble, Brendon; Mccormick, Adele; Basnett, Pooja. - In: FRONTIERS IN NANOTECHNOLOGY. - ISSN 2673-3013. - ELETTRONICO. - 5:(2023). [10.3389/fnano.2023.1243056]
Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles
Marcello, Elena;
2023
Abstract
Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.File | Dimensione | Formato | |
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https://hdl.handle.net/11583/2982075