Deterministic lateral displacement (DLD) is a passive separation method that separates particles by hydrodynamic size. This label-free method is a promising technique for cell separation because of its high size resolution and insensitivity to flow rate. Development of capillary-driven microfluidic technologies allows microfluidic devices to be operated without any external power for fluid pumping, lowering their total cost and complexity. Herein, we develop and test a DLD-based particle and cell sorting method that is driven entirely by capillary pressure. We show microchip self-filling, flow focusing, flow stability, and capture of separated particles. We achieve separation efficiency of 92% for particle-particle separation and more than 99% efficiency for cell-particle separation. The high performance of driven flow and separation along with simplicity of the operation and setup make it a valuable candidate for point-of-care devices.
Pumpless deterministic lateral displacement separation using a paper capillary wick / Aghajanloo, B.; Ejeian, F.; Frascella, F.; Marasso, S. L.; Cocuzza, M.; Tehrani, A. F.; Nasr Esfahani, M. H.; Inglis, D. W.. - In: LAB ON A CHIP. - ISSN 1473-0197. - ELETTRONICO. - 23:8(2023), pp. 2106-2112. [10.1039/d3lc00039g]
Pumpless deterministic lateral displacement separation using a paper capillary wick
Aghajanloo B.;Frascella F.;Marasso S. L.;Cocuzza M.;
2023
Abstract
Deterministic lateral displacement (DLD) is a passive separation method that separates particles by hydrodynamic size. This label-free method is a promising technique for cell separation because of its high size resolution and insensitivity to flow rate. Development of capillary-driven microfluidic technologies allows microfluidic devices to be operated without any external power for fluid pumping, lowering their total cost and complexity. Herein, we develop and test a DLD-based particle and cell sorting method that is driven entirely by capillary pressure. We show microchip self-filling, flow focusing, flow stability, and capture of separated particles. We achieve separation efficiency of 92% for particle-particle separation and more than 99% efficiency for cell-particle separation. The high performance of driven flow and separation along with simplicity of the operation and setup make it a valuable candidate for point-of-care devices.File | Dimensione | Formato | |
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Paper wick-driven microfluidic platform for particle separation.pdf
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d3lc00039g.pdf
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https://hdl.handle.net/11583/2981629