Immobilization of LDH enzyme on mesoporous silica with FDH as a cofactor regeneration system for the evaluation of novel cancer drugs

Tumor cells are characterized by the uncontrolled growth and the production of high amount of lactic acid via the Cori cycle (anaerobic conditions). The production of lactic acid is catalyzed by the A form of Lactate dehydrogenase (LDH A). Thus, the immobilization of LDH has shown to serve as a simple method to study the inhibition by means of anticancer inhibitors. In our previous work, we successfully immobilized the Formate dehydrogenase (FDH) on synthesized mesoporous silica, Mesoporous Cellular Foams-type (MCF). In this scenario, the similarities between the protein structure of FDH and LDH allows predicting a good performance of MCF as support for LDH enzyme.