Bioactive glass (BG) represents a promising biomaterial for bone healing; here injectable BG pastes biological properties were improved by the addition of gelatin or chitosan, as well as mechanical resistance was enhanced by adding 10 or 20 wt% 3-Glycidyloxypropyl trimethoxysilane (GPTMS) cross-linker. Composite pastes exhibited bioactivity as apatite formation was observed by Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD) after 14 days immersion in simulated body fluid (SBF); moreover, polymers did not enhance degradability as weight loss was >10% after 30 days in physiological conditions. BG-gelatin-20 wt% GPTMS composites demonstrated the highest compressive strength (4.8 ± 0.5 MPa) in comparison with the bulk control paste made of 100% BG in water (1.9 ± 0.1 MPa). Cytocompatibility was demonstrated towards human mesenchymal stem cells (hMSC), osteoblasts progenitors, and endothelial cells. The presence of 20 wt% GPTMS conferred antibacterial properties thus inhibiting the joint pathogens Staphylococcus aureus and Staphylococcus epidermidis infection. Finally, hMSC osteogenesis was successfully supported in a 3D model as demonstrated by alkaline phosphatase release and osteogenic genes expression.
Enhancing mechanical properties and biological performances of injectable bioactive glass by gelatin and chitosan for bone small defect repair / Sohrabi, M.; Yekta, B. E.; Rezaie, H.; Naimi-Jamal, M. R.; Kumar, A.; Cochis, A.; Miola, M.; Rimondini, L.. - In: BIOMEDICINES. - ISSN 2227-9059. - ELETTRONICO. - 8:12(2020), pp. 1-19.
|Titolo:||Enhancing mechanical properties and biological performances of injectable bioactive glass by gelatin and chitosan for bone small defect repair|
|Data di pubblicazione:||2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/biomedicines8120616|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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