The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.

Raman on suspended DNA: Novel super-hydrophobic approach for structural studies / Marini, M.; Allione, M.; Torre, B.; Moretti, M.; Limongi, T.; Tirinato, L.; Giugni, A.; Das, G.; di Fabrizio, E.. - In: MICROELECTRONIC ENGINEERING. - ISSN 0167-9317. - 175:(2017), pp. 38-42. [10.1016/j.mee.2016.12.016]

Raman on suspended DNA: Novel super-hydrophobic approach for structural studies

Marini M.;Torre B.;Limongi T.;di Fabrizio E.
2017

Abstract

The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11583/2852814