Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing. © 2012 American Chemical Society.

Direct imaging of DNA fibers: The visage of double helix / Gentile, F.; Moretti, M.; Limongi, T.; Falqui, A.; Bertoni, G.; Scarpellini, A.; Santoriello, S.; Maragliano, L.; Proietti Zaccaria, R.; Di Fabrizio, E.. - In: NANO LETTERS. - ISSN 1530-6984. - 12:12(2012), pp. 6453-6458. [10.1021/nl3039162]

Direct imaging of DNA fibers: The visage of double helix

Limongi T.;Di Fabrizio E.
2012

Abstract

Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing. © 2012 American Chemical Society.
2012
File in questo prodotto:
File Dimensione Formato  
Nanoletters_2012.pdf

non disponibili

Tipologia: 2a Post-print versione editoriale / Version of Record
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 1.42 MB
Formato Adobe PDF
1.42 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2852788