Decellularized extracellular matrix is one of the most promising biological scaffold supporting in vitro tissue growth and in vivo tissue regeneration in both preclinical research and clinical practice. In case of thick tissues or even organs, conventional static decellularization methods based on chemical or enzymatic treatments are not effective in removing the native cellular material without affecting the extracellular matrix. To overcome this limitation, dynamic decellularization methods, mostly based on perfusion and agitation, have been proposed. In this study, we developed a low-cost scalable 3D-printed sample-holder for agitation-based decellularization purposes, designed for treating multiple specimens simultaneously and for improving efficiency, homogeneity and reproducibility of the decellularization treatment with respect to conventional agitation-based approaches. In detail, the proposed sample-holder is able to house up to four specimens and, immersed in the decellularizing solution within a beaker placed on a magnetic stirrer, to expose them to convective flow, enhancing the solution transport through the specimens while protecting them. Computational fluid dynamics analyses were performed to investigate the fluid phenomena establishing within the beaker and to support the sample-holder design. Exploratory biological tests performed on human skin specimens demonstrated that the sample-holder reduces process duration and increases treatment homogeneity and reproducibility.

A low-cost scalable 3D-printed sample-holder for agitation-based decellularization of biological tissues / Carbonaro, Dario; Putame, Giovanni; Castaldo, Clotilde; Meglio, Franca Di; Siciliano, Katia; Belviso, Immacolata; Romano, Veronica; Sacco, Anna Maria; Schonauer, Fabrizio; Montagnani, Stefania; Audenino, Alberto L.; Morbiducci, Umberto; Gallo, Diego; Massai, Diana. - In: MEDICAL ENGINEERING & PHYSICS. - ISSN 1350-4533. - ELETTRONICO. - 85:(2020), pp. 7-15. [10.1016/j.medengphy.2020.09.006]

A low-cost scalable 3D-printed sample-holder for agitation-based decellularization of biological tissues

Carbonaro, Dario;Putame, Giovanni;Siciliano, Katia;Audenino, Alberto L.;Morbiducci, Umberto;Gallo, Diego;Massai, Diana
2020

Abstract

Decellularized extracellular matrix is one of the most promising biological scaffold supporting in vitro tissue growth and in vivo tissue regeneration in both preclinical research and clinical practice. In case of thick tissues or even organs, conventional static decellularization methods based on chemical or enzymatic treatments are not effective in removing the native cellular material without affecting the extracellular matrix. To overcome this limitation, dynamic decellularization methods, mostly based on perfusion and agitation, have been proposed. In this study, we developed a low-cost scalable 3D-printed sample-holder for agitation-based decellularization purposes, designed for treating multiple specimens simultaneously and for improving efficiency, homogeneity and reproducibility of the decellularization treatment with respect to conventional agitation-based approaches. In detail, the proposed sample-holder is able to house up to four specimens and, immersed in the decellularizing solution within a beaker placed on a magnetic stirrer, to expose them to convective flow, enhancing the solution transport through the specimens while protecting them. Computational fluid dynamics analyses were performed to investigate the fluid phenomena establishing within the beaker and to support the sample-holder design. Exploratory biological tests performed on human skin specimens demonstrated that the sample-holder reduces process duration and increases treatment homogeneity and reproducibility.
File in questo prodotto:
File Dimensione Formato  
2020_Carbonaro_ALowCostScalable3DprintedSampleHolderForAgitationBasedDecellularizationOfBiologicalTissues.pdf

non disponibili

Tipologia: 2a Post-print versione editoriale / Version of Record
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 3.64 MB
Formato Adobe PDF
3.64 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
JJBE_3553_edit_report.pdf

Open Access dal 20/09/2021

Tipologia: 1. Preprint / submitted version [pre- review]
Licenza: Creative commons
Dimensione 1.99 MB
Formato Adobe PDF
1.99 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2846727