PORTO @ 
Archivio Istituzionale della Ricerca
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishWe report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.
Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices / Rizzo, Riccardo; Alvaro, Maria; Danz, Norbert; Napione, Lucia; Descrovi, Emiliano; Schmieder, Stefan; Sinibaldi, Alberto; Chandrawati, Rona; Rana, Subinoy; Munzert, Peter; Schubert, Thomas; Maillart, Emmanuel; Anopchenko, Aleksei; Rivolo, Paola; Mascioletti, Alessandro; Sonntag, Frank; Stevens, Molly M.; Bussolino, Federico; Michelotti, Francesco. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 255(2018), pp. 2143-2150.
| Titolo: | Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices |
| Autori: | |
| Data di pubblicazione: | 2018 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.snb.2017.09.018 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| biloba1_ SENS_ACTUATOR B_ final_1-s2.0-S0925400517316829-main.pdf | 2. Post-print / Author's Accepted Manuscript | Non Pubblico - Accesso privato/ristretto | Administrator Richiedi una copia |
http://hdl.handle.net/11583/2687025
Copyright © 2020