In this work, silver decorated porous silicon membranes integrated in a polydimethylsiloxane multi-chamber microfluidic chip were functionalized with DNA-probes and used for the detection of miRNA by Surface-enhanced Raman Scattering analysis. An innovative biological protocol has been designed: the probe was divided in two short pieces that interact before and after the miRNA incubation. The optofluidic biosensor was applied for the label-free detection of miRNA sequences at in vivo concentrations.
SERS-active metal-dielectric nanostructures integrated in microfluidic devices for ultra-sensitive label-free miRNA detection / CHIADO', ALESSANDRO; NOVARA, CHIARA; LAMBERTI, ANDREA; GEOBALDO, FRANCESCO; GIORGIS, FABRIZIO; RIVOLO, PAOLA. - In: PROCEDIA TECHNOLOGY. - ISSN 2212-0173. - ELETTRONICO. - 27(2017), pp. 37-38.
|Titolo:||SERS-active metal-dielectric nanostructures integrated in microfluidic devices for ultra-sensitive label-free miRNA detection|
|Data di pubblicazione:||2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.protcy.2017.04.018|
|Appare nelle tipologie:||1.1 Articolo in rivista|