A well-organized immobilization of bio-receptors is a crucial goal in biosensing, especially to achieve high reproducibility, sensitivity and specificity. These requirements are usually attained with a controlled chemical/biochemical functionalization that creates a stable layer on a sensor surface. In this work, a chemical modification protocol for silicon-based surfaces to be applied in biosensing devices is presented. An anhydrous silanization step through 3-aminopropylsilane (APTES), followed by a further derivatization with succinic anhydride (SA), is optimized to generate an ordered flat layer of carboxylic groups. The properties of APTES/SA modified surface were compared with a functionalization in which glutaraldehyde (GA) is used as crosslinker instead of SA, in order to have a comparison with an established and largely applied procedure. Moreover, a functionalization based on the controlled deposition of a plasma polymerized acrylic acid (PPAA) thin film was used as a reference for carboxylic reactivity. Advantages and drawbacks of the considered methods are highlighted, through physico-chemical characterizations (OCA, XPS, and AFM) and by means of a functional Protein G/Antibody immunoassay. These analyses reveal that the most homogeneous, reproducible and active surface is achieved by using the optimized APTES/SA coupling.
Optimization and characterization of a homogeneous carboxylic surface functionalization for silicon-based biosensing / Chiadò, Alessandro; Palmara, Gianluca; Ricciardi, Serena; Frascella, Francesca; Castellino, Micaela; Tortello, Mauro; Ricciardi, Carlo; Rivolo, Paola. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - ELETTRONICO. - 143(2016), pp. 252-259.
|Titolo:||Optimization and characterization of a homogeneous carboxylic surface functionalization for silicon-based biosensing|
|Data di pubblicazione:||2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.colsurfb.2016.03.048|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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