A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves / Frascella, Francesca; Ricciardi, Serena; Rivolo, Paola; Moi, Valeria; Giorgis, Fabrizio; Descrovi, Emiliano; Michelotti, F.; Munzert, P.; Danz, N.; Napione, L.; Alvaro, M.; Bussolino, F.. - In: SENSORS. - ISSN 1424-8220. - ELETTRONICO. - 13:(2013), pp. 2011-2022. [10.3390/s130202011]

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

FRASCELLA, FRANCESCA;RICCIARDI, SERENA;RIVOLO, PAOLA;MOI, VALERIA;GIORGIS, FABRIZIO;DESCROVI, EMILIANO;Napione L.;
2013

Abstract

A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2508679
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