Archivio Istituzionale della RicercaA fluorescent dielectric multilayer is exploited for label-free sensing in aqueous microenvironment. Fluorescence is laser-excited and collected through prism-coupling to a surface electromagnetic mode, also known as Bloch surface waves (BSW) localized at the interface between the multilayer and the outer aqueous medium. By detecting the spectral changes of the BSW-coupled light emission due to an external perturbation of the refractive index (Dn), a sensitivity of 2500 nm/RIU and a limit of detection down to Dn=3 10^-6 are obtained.
Surface label-free sensing by means of a fluorescent multilayered photonic structure / Descrovi, Emiliano; Frascella, Francesca; Ballarini, M.; Moi, Valeria; Lamberti, Andrea; Michelotti, F.; Giorgis, Fabrizio; Pirri, Candido. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - 101:(2012), p. 131105. [10.1063/1.4754615]
Surface label-free sensing by means of a fluorescent multilayered photonic structure
DESCROVI, EMILIANO;FRASCELLA, FRANCESCA;MOI, VALERIA;LAMBERTI, ANDREA;GIORGIS, FABRIZIO;PIRRI, Candido
2012
Abstract
A fluorescent dielectric multilayer is exploited for label-free sensing in aqueous microenvironment. Fluorescence is laser-excited and collected through prism-coupling to a surface electromagnetic mode, also known as Bloch surface waves (BSW) localized at the interface between the multilayer and the outer aqueous medium. By detecting the spectral changes of the BSW-coupled light emission due to an external perturbation of the refractive index (Dn), a sensitivity of 2500 nm/RIU and a limit of detection down to Dn=3 10^-6 are obtained.
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https://hdl.handle.net/11583/2502541
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