The effects of Stöber silica nanoparticles on neuronal survival, proliferation, and on the underlying perturbations in calcium homeostasis are investigated on the welldifferentiated neuronal cell line GT1-7. The responses to nanoparticles 50 and 200 nm in diameter are compared. The 50-nm silica affects neuronal survival/proliferation in a dose-dependent way, by stimulating apoptotic processes. In contrast, 200-nm silica does not show any toxic effects even at relatively high concentrations (292 μ g mL − 1 ). To identify the mechanisms underlying these effects, the changes in intracellular calcium concentration elicited by acute and chronic administration of the two silica nanoparticles are analyzed. The 50-nm silica nanoparticles at toxic concentrations generate huge and long-lasting increases in intracellular calcium, whereas 200-nm silica only induces transient signals of much lower amplitude. These fi ndings provide the fi rst evidence that silica nanoparticles can induce toxic effects on neuronal cells in a size-dependent way, and that these effects are related to the degree of perturbation of calcium homeostasis.

Interaction of Spherical Silica Nanoparticleswith Neuronal Cells: Size-DependentToxicity and Perturbation of Calcium Homeostasis / Ariano, P.; Zamburlin, P.; Gilardino, A.; Mortera, RENATO SILVIO; Onida, Barbara; Tomatis, M.; Ghiazza, M.; Fubini, B.; Lovisolo, D.. - In: SMALL. - ISSN 1613-6810. - STAMPA. - 7:6(2011), pp. 766-774. [10.1002/smll.201002287]

Interaction of Spherical Silica Nanoparticleswith Neuronal Cells: Size-DependentToxicity and Perturbation of Calcium Homeostasis

MORTERA, RENATO SILVIO;ONIDA, BARBARA;
2011

Abstract

The effects of Stöber silica nanoparticles on neuronal survival, proliferation, and on the underlying perturbations in calcium homeostasis are investigated on the welldifferentiated neuronal cell line GT1-7. The responses to nanoparticles 50 and 200 nm in diameter are compared. The 50-nm silica affects neuronal survival/proliferation in a dose-dependent way, by stimulating apoptotic processes. In contrast, 200-nm silica does not show any toxic effects even at relatively high concentrations (292 μ g mL − 1 ). To identify the mechanisms underlying these effects, the changes in intracellular calcium concentration elicited by acute and chronic administration of the two silica nanoparticles are analyzed. The 50-nm silica nanoparticles at toxic concentrations generate huge and long-lasting increases in intracellular calcium, whereas 200-nm silica only induces transient signals of much lower amplitude. These fi ndings provide the fi rst evidence that silica nanoparticles can induce toxic effects on neuronal cells in a size-dependent way, and that these effects are related to the degree of perturbation of calcium homeostasis.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2381241
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