Owing to their pivotal role as expression regulators, microRNAs (miRNAs) have different physiological roles and can be involved in the onset and progression of several diseases. For this reason, their altered presence can be predictive of a pathological state. Furthermore, the ubiquitous presence of these short RNA sequences in basically all body tissues and fluids makes them elite candidates as biomarkers. With this concept in mind, it is fundamental to have effective, sensitive, and selective techniques to perform their accurate detection and profiling. The goal of this chapter is to summarize the pros and cons of some of the available detection approaches used for miRNA profiling, starting from the most common unamplified/amplified probe-based methods to the advanced techniques based on biosensors and bioassays.

Detection methodologies for microRNA biomarker profiling / Bertone, Sofia; Calmo, Roberta; Chiado', Alessandro - In: MicroRNA: From Bench to BedsideELETTRONICO. - [s.l] : Elsevier, 2022. - ISBN 9780323897747. - pp. 217-240 [10.1016/B978-0-323-89774-7.00029-7]

Detection methodologies for microRNA biomarker profiling

Bertone, Sofia;Calmo, Roberta;Chiado', Alessandro
2022

Abstract

Owing to their pivotal role as expression regulators, microRNAs (miRNAs) have different physiological roles and can be involved in the onset and progression of several diseases. For this reason, their altered presence can be predictive of a pathological state. Furthermore, the ubiquitous presence of these short RNA sequences in basically all body tissues and fluids makes them elite candidates as biomarkers. With this concept in mind, it is fundamental to have effective, sensitive, and selective techniques to perform their accurate detection and profiling. The goal of this chapter is to summarize the pros and cons of some of the available detection approaches used for miRNA profiling, starting from the most common unamplified/amplified probe-based methods to the advanced techniques based on biosensors and bioassays.
2022
9780323897747
MicroRNA: From Bench to Bedside
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2973411