Archivio Istituzionale della RicercaWhispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was numerically modelled and optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
Whispering Gallery Mode Aptasensors for Detection of Blood Proteins / Pasquardini, L.; Berneschi, S.; Barucci, A.; Cosi, F.; Insinna, Massimiliano; Lunelli, L.; Nunzi Conti, G.; Pederzolli, C.; Salvadori, Simone; Soria, S.. - ELETTRONICO. - (2013), pp. 1-8. (Intervento presentato al convegno SPIE BiOS tenutosi a San Francisco, California, United States) [10.1117/12.2001987].
Whispering Gallery Mode Aptasensors for Detection of Blood Proteins
SALVADORI, SIMONE;
2013
Abstract
Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was numerically modelled and optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11583/2761073
Attenzione
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo