Na/K-ATPase powder samples were analyzed, both in lyophilized and rehydrated states, by a latest generation Raman spectrometer mounting Ultra Low Frequency (ULF) filters that allowed investigating even the lower frequencies (<500 cm^-1 = 15 THz). While several known peaks, which are assigned in the literature to molecular vibrations of chemical groups or amino acids, were recognised in the 500–3500 cm^-1 range, the analysis of the sub–500 cm^-1 region, performed with two different resolutions, allowed finding unassigned peaks. Such low-frequency peaks are apparently unknown in the current literature for Na/K-ATPase. Recent studies suggested that low-frequency collective vibrations that involve large protein portions are strictly correlated to protein functions. Thus, the present results could be of interest with regard to possible connections between low-frequency mechanical vibrations and the operating mechanism of the sodium-potassium pump.

Raman spectroscopy of Na/K-ATPase with special focus on low-frequency vibrations / Lacidogna, Giuseppe; Piana, Gianfranco; Bassani, Andrea; Carpinteri, Alberto. - In: VIBRATIONAL SPECTROSCOPY. - ISSN 0924-2031. - STAMPA. - 92:(2017), pp. 298-301. [10.1016/j.vibspec.2017.08.002]

Raman spectroscopy of Na/K-ATPase with special focus on low-frequency vibrations

LACIDOGNA, GIUSEPPE;PIANA, GIANFRANCO;BASSANI, ANDREA;CARPINTERI, ALBERTO
2017

Abstract

Na/K-ATPase powder samples were analyzed, both in lyophilized and rehydrated states, by a latest generation Raman spectrometer mounting Ultra Low Frequency (ULF) filters that allowed investigating even the lower frequencies (<500 cm^-1 = 15 THz). While several known peaks, which are assigned in the literature to molecular vibrations of chemical groups or amino acids, were recognised in the 500–3500 cm^-1 range, the analysis of the sub–500 cm^-1 region, performed with two different resolutions, allowed finding unassigned peaks. Such low-frequency peaks are apparently unknown in the current literature for Na/K-ATPase. Recent studies suggested that low-frequency collective vibrations that involve large protein portions are strictly correlated to protein functions. Thus, the present results could be of interest with regard to possible connections between low-frequency mechanical vibrations and the operating mechanism of the sodium-potassium pump.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2689587
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