Archivio Istituzionale della RicercaIn this work, silver decorated porous silicon membranes integrated in a polydimethylsiloxane multi-chamber microfluidic chip were functionalized with DNA-probes and used for the detection of miRNA by Surface-enhanced Raman Scattering analysis. An innovative biological protocol has been designed: the probe was divided in two short pieces that interact before and after the miRNA incubation. The optofluidic biosensor was applied for the label-free detection of miRNA sequences at in vivo concentrations.
SERS-active metal-dielectric nanostructures integrated in microfluidic devices for ultra-sensitive label-free miRNA detection / Chiado', Alessandro; Novara, Chiara; Lamberti, Andrea; Geobaldo, Francesco; Giorgis, Fabrizio; Rivolo, Paola. - In: PROCEDIA TECHNOLOGY. - ISSN 2212-0173. - ELETTRONICO. - 27:(2017), pp. 37-38. [10.1016/j.protcy.2017.04.018]
SERS-active metal-dielectric nanostructures integrated in microfluidic devices for ultra-sensitive label-free miRNA detection
CHIADO', ALESSANDRO;NOVARA, CHIARA;LAMBERTI, ANDREA;GEOBALDO, FRANCESCO;GIORGIS, FABRIZIO;RIVOLO, PAOLA
2017
Abstract
In this work, silver decorated porous silicon membranes integrated in a polydimethylsiloxane multi-chamber microfluidic chip were functionalized with DNA-probes and used for the detection of miRNA by Surface-enhanced Raman Scattering analysis. An innovative biological protocol has been designed: the probe was divided in two short pieces that interact before and after the miRNA incubation. The optofluidic biosensor was applied for the label-free detection of miRNA sequences at in vivo concentrations.
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https://hdl.handle.net/11583/2684061
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