In this paper a device, based on urease-loaded microspheres, is presented. The first task of this work was the optimization of a procedure for the alginate microspheres realization, having a radius as close as possible to the optimal one necessary to achieve the maximum enzyme exploitation. This optimal radius was calculated theoretically through a mathematical model which describes the concentration of substrate (urea) inside the microspheres on the assumption of a diffusion-reaction mechanism. The enzyme-loaded microspheres were successfully tested in a prototypal device aimed at the depletion of urea from a circulating fluid simulating blood flow: the results showed that urea concentration in the circulating fluid drops down to less than 25% of the initial value after 5 h.

Urease loaded alginate microspheres for blood purification / Ciofani, Gianni; Cascone, Maria Grazia; Serino, Lorenzo Pio; Lazzeri, Luigi. - In: JOURNAL OF MICROENCAPSULATION. - ISSN 0265-2048. - 25:8(2008), pp. 569-576. [10.1080/02652040802081227]

Urease loaded alginate microspheres for blood purification

CIOFANI, GIANNI;
2008

Abstract

In this paper a device, based on urease-loaded microspheres, is presented. The first task of this work was the optimization of a procedure for the alginate microspheres realization, having a radius as close as possible to the optimal one necessary to achieve the maximum enzyme exploitation. This optimal radius was calculated theoretically through a mathematical model which describes the concentration of substrate (urea) inside the microspheres on the assumption of a diffusion-reaction mechanism. The enzyme-loaded microspheres were successfully tested in a prototypal device aimed at the depletion of urea from a circulating fluid simulating blood flow: the results showed that urea concentration in the circulating fluid drops down to less than 25% of the initial value after 5 h.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/2621476
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