Biochip-integrable sorting and separation of micron-sized particles have an increasing importance in biomedical diagnostics, biochemical analyses, food and chemical processing, and environmental assessment. By employing the unique characteristics of microscale flow phenomena, various techniques have been established for fast and accurate separation, and to sort cells or particles in a continuous manner. As in classical separation procedures, the biochip-integrable size-fractionation of particles or cells could be realized by passive or active way. Passive procedures, which do not require external force-field, utilize the interaction between particles-particle, flow-particle, and the channel structure-particle to separate different-sized particles. Meanwhile, the active separation techniques make use of external force-field in various forms. This doctoral thesis provides a novel biochip-integrable pathogen detection device (Flow Through Nematode Filter, FTNF), and a novel application of an asymmetric column structure, which called deterministic lateral displacement (DLD) device. The working principles are explained in detail, and performances of the devices are discussed with the results of the measurements. The main target of this represented work is applications in medicine and biomedical research but we are also open for other application areas. The use of these simple microfluidic devices will make it possible to extend the use of cell-sorting to the point of care, closer to the patient at the clinic or in the field.
Biochip-Integrable Microfluidic Particle Separation Techniques for Biomedical Use / Laki, ANDRAS JOZSEF. - (2015). [10.6092/polito/porto/2585574]
Biochip-Integrable Microfluidic Particle Separation Techniques for Biomedical Use
LAKI, ANDRAS JOZSEF
2015
Abstract
Biochip-integrable sorting and separation of micron-sized particles have an increasing importance in biomedical diagnostics, biochemical analyses, food and chemical processing, and environmental assessment. By employing the unique characteristics of microscale flow phenomena, various techniques have been established for fast and accurate separation, and to sort cells or particles in a continuous manner. As in classical separation procedures, the biochip-integrable size-fractionation of particles or cells could be realized by passive or active way. Passive procedures, which do not require external force-field, utilize the interaction between particles-particle, flow-particle, and the channel structure-particle to separate different-sized particles. Meanwhile, the active separation techniques make use of external force-field in various forms. This doctoral thesis provides a novel biochip-integrable pathogen detection device (Flow Through Nematode Filter, FTNF), and a novel application of an asymmetric column structure, which called deterministic lateral displacement (DLD) device. The working principles are explained in detail, and performances of the devices are discussed with the results of the measurements. The main target of this represented work is applications in medicine and biomedical research but we are also open for other application areas. The use of these simple microfluidic devices will make it possible to extend the use of cell-sorting to the point of care, closer to the patient at the clinic or in the field.File | Dimensione | Formato | |
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PhD_dissertation_Laki.pdf
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https://hdl.handle.net/11583/2585574
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